human bmp6 elisa kit  (Cusabio)

Journal: Biomedicines

Article Title: Reduced Plasma Bone Morphogenetic Protein 6 Levels in Sepsis and Septic Shock Patients

doi: 10.3390/biomedicines12081682

Figure Lengend Snippet: BMP6 in plasma of controls, patients with SIRS, patients with sepsis, and patients with septic shock. ** p < 0.01.

Article Snippet: BMP6 levels were measured in duplicate using the human BMP6 ELISA Kit from Invitrogen (ThermoFisher Scientific, Carlsbad, CA, USA) as recommended by the manufacturer.

Techniques: Clinical Proteomics

Journal: Biomedicines

Article Title: Reduced Plasma Bone Morphogenetic Protein 6 Levels in Sepsis and Septic Shock Patients

doi: 10.3390/biomedicines12081682

Figure Lengend Snippet: Correlation coefficient (r) and p -values for plasma BMP6, ferritin, iron, and transferrin levels, and their associations with leukocyte numbers and clinical markers of inflammation in SIRS/sepsis/septic shock patients without liver cirrhosis. Statistical test used: Spearman correlation.

Article Snippet: BMP6 levels were measured in duplicate using the human BMP6 ELISA Kit from Invitrogen (ThermoFisher Scientific, Carlsbad, CA, USA) as recommended by the manufacturer.

Techniques: Clinical Proteomics

Journal: Biomedicines

Article Title: Reduced Plasma Bone Morphogenetic Protein 6 Levels in Sepsis and Septic Shock Patients

doi: 10.3390/biomedicines12081682

Figure Lengend Snippet: BMP6 and iron in plasma of patients with sepsis/septic shock stratified for underlying diseases and causes of sepsis/septic shock. ( a ) Plasma BMP6 levels of sepsis/septic shock patients with liver cirrhosis, pancreatitis, or cholangiosepsis. ( b ) Serum iron levels of SIRS/sepsis/septic shock patients with liver cirrhosis, pancreatitis, or cholangiosepsis. Outliers are depicted as circles (mild outliers) or asterisks (extreme outliers).

Article Snippet: BMP6 levels were measured in duplicate using the human BMP6 ELISA Kit from Invitrogen (ThermoFisher Scientific, Carlsbad, CA, USA) as recommended by the manufacturer.

Techniques: Clinical Proteomics

Journal: Biomedicines

Article Title: Reduced Plasma Bone Morphogenetic Protein 6 Levels in Sepsis and Septic Shock Patients

doi: 10.3390/biomedicines12081682

Figure Lengend Snippet: BMP6 in plasma of controls as well as sepsis/septic shock patients stratified for SARS-CoV-2. ( a ) Plasma BMP6 levels of the 23 patients with SARS-CoV-2 infection in contrast to patients not infected by this virus. ( b ) Plasma BMP6 levels of the 23 patients with SARS-CoV-2 infection in contrast to the 43 healthy controls.

Article Snippet: BMP6 levels were measured in duplicate using the human BMP6 ELISA Kit from Invitrogen (ThermoFisher Scientific, Carlsbad, CA, USA) as recommended by the manufacturer.

Techniques: Clinical Proteomics, Infection, Virus

Journal: Biomedicines

Article Title: Reduced Plasma Bone Morphogenetic Protein 6 Levels in Sepsis and Septic Shock Patients

doi: 10.3390/biomedicines12081682

Figure Lengend Snippet: Plasma BMP6 levels of sepsis/septic shock and SIRS patients with/without dialysis, ventilation and vasopressor therapy. The number of patients treated is given in “N”, the percent relative to the whole cohort in brackets, and the respective p -values are listed.

Article Snippet: BMP6 levels were measured in duplicate using the human BMP6 ELISA Kit from Invitrogen (ThermoFisher Scientific, Carlsbad, CA, USA) as recommended by the manufacturer.

Techniques: Clinical Proteomics

Journal: Biomedicines

Article Title: Reduced Plasma Bone Morphogenetic Protein 6 Levels in Sepsis and Septic Shock Patients

doi: 10.3390/biomedicines12081682

Figure Lengend Snippet: BMP6 in plasma of patients with sepsis/septic shock stratified for type of bacterial infection.

Article Snippet: BMP6 levels were measured in duplicate using the human BMP6 ELISA Kit from Invitrogen (ThermoFisher Scientific, Carlsbad, CA, USA) as recommended by the manufacturer.

Techniques: Clinical Proteomics, Infection

Journal: Biomedicines

Article Title: Reduced Plasma Bone Morphogenetic Protein 6 Levels in Sepsis and Septic Shock Patients

doi: 10.3390/biomedicines12081682

Figure Lengend Snippet: BMP6 and ferritin in blood of survivors and non-survivors in patients with sepsis/septic shock. ( a ) BMP6 plasma of survivors and non-survivors in patients with sepsis/septic shock. ( b ) Ferritin of survivors and non-survivors in patients with SIRS/sepsis/septic shock, excluding patients with liver cirrhosis. Outliers are depicted as circles (mild outliers) or asterisks (extreme outliers).

Article Snippet: BMP6 levels were measured in duplicate using the human BMP6 ELISA Kit from Invitrogen (ThermoFisher Scientific, Carlsbad, CA, USA) as recommended by the manufacturer.

Techniques: Clinical Proteomics

Journal: eBioMedicine

Article Title: H3K27me3-modulated Hofbauer cell BMP2 signalling enhancement compensates for shallow trophoblast invasion in preeclampsia

doi: 10.1016/j.ebiom.2023.104664

Figure Lengend Snippet: BMP2 transcriptionally activates an anti-preeclampsia transcription program and BMP6 is increased in the serum of preeclamptic patients . a-g, HTR8/SVneo cells were treated with or without 25 ng/mL BMP2 for 6 h or 24 h prior to RNA-seq. a , PCA showing the separation between samples from the four experimental groups. b , Heatmap depicting DEG (adjusted p value < 0.05) signatures: “6 h specific” refers to genes only differentially expressed after 6 h BMP2 treatment; “24 h specific” for genes only differentially expressed after 24 h BMP2 treatment; “Common” for genes differentially expressed after both 6 h and 24 h BMP2 treatment, and sharing the same changing trend, and “Variable in common” for genes with opposite changing trends. c-e , Waterfall plots showing “Common” DEGs ( c ), “6 h specific” DEGs ( d ), and “24 h specific” DEGs ( e ). The gene lists are ranked by log2 fold change and all dots indicate DEGs. Red dots indicate DEGs with log2 fold change >2 ( c and e ) or log2 fold change >1 ( d ). Blue dots indicate DEGs with log2 fold change < −2 ( c and e ) or log2 fold change < −1 ( d ). Cutpoints of 1 and 2 for log2 fold change were used in our RNAseq analysis, representing 2-fold and 4-fold changes in gene expression respectively. These thresholds, chosen based on typical standards and our specific experimental design, strike a balance between identifying meaningful biological changes and controlling false positives. f - g , Dot plots of significantly enriched pathways ( f ) and GO terms ( g ). Dot size represents the number of DEGs from a particular pathway or GO term (count). h , HTR8/SVneo cells were treated with or without 25 ng/mL BMP2 for the indicated durations, followed by qPCR analysis of BMP6 levels using GAPDH as the reference gene. i , HTR8/SVneo cells were treated with or without the indicated BMP2 doses for 6 h, followed by qPCR analysis of BMP6 levels. j, BMP6 concentrations in the serum of healthy and preeclamptic pregnant women assayed by ELISA (n = 41 vs. 26). k , ROC curve for serum BMP6 level as a diagnostic marker for PE. AUC with 95% CI is labeled. Each dot donates one sample and quantitative results are expressed as the mean with 95% CI. p values by two-tailed Student's t test are labeled in ( h , i ) and p value by Mann–Whitney U test is labeled in ( j ).

Article Snippet: Sera were immediately isolated, sub-bottled, and stored at −80 °C until being assayed using Human BMP6 and BMP2 ELISA kit (LSBio, LS-F4538; R&D, DBP200).

Techniques: RNA Sequencing Assay, Expressing, Enzyme-linked Immunosorbent Assay, Diagnostic Assay, Marker, Labeling, Two Tailed Test, MANN-WHITNEY

Journal: eBioMedicine

Article Title: H3K27me3-modulated Hofbauer cell BMP2 signalling enhancement compensates for shallow trophoblast invasion in preeclampsia

doi: 10.1016/j.ebiom.2023.104664

Figure Lengend Snippet: BMP6 mediates basal and BMP2-induced human trophoblast invasion and vascular mimicry . a-b, HTR8/SVneo cells were treated with or without 50 ng/mL BMP6 for 36 h, followed by analysis of cell invasiveness ( a ) and endothelial-like tube formation ( b ). The left panel shows representative images, and the right panel shows summarized quantitative results. Scale bar, 200 μm. c-e, HTR8/SVneo cells were treated with or without 50 ng/mL BMP6 for 6 h or 24 h, followed by RNA-seq. c , PCA showing the separation between samples. d , Heatmap depicting DEG (adjusted p value < 0.05) signatures of each group. The adjusted p value was obtained through the Benjamini-Hochberg procedure, which corrects for multiple comparisons by ranking and recalibrating individual p values, thereby minimizing false positives. e , Dot plots of significantly enriched GO terms. Dot size represents the number of DEGs from a particular term (count). f-h , Cells were transfected for 48 h with 20 nM non-targeting control siRNA or siRNA targeting BMP6 prior to treatment with or without 25 ng/mL BMP2 for 6 or 36 h. f , BMP6 mRNA levels were examined by qPCR 6 h after BMP2 treatment. g , Cell invasiveness was examined with Transwell assay. The upper panel shows summarized quantitative results; the lower panel shows representative images. Scale bar, 200 μm. h , The vascular mimicry phenotype of HTR8/SVneo cells was examined by an endothelial-like tube formation assay. The upper panels show summarized quantitative total branching points and total tube length results, and the lower panels show representative images. Scale bar, 200 μm. i , Extravillous explants from first-trimester villi were transfected with 20 nM non-targeting control siRNA or siRNA targeting BMP6 prior to treatment with or without 25 ng/mL BMP2. The migration distance of villous tips was qualified in the right panel. Scale bar, 100 μm. Each dot donates one sample and data is expressed as the mean with 95% CI. p values by two-tailed Student's t test are labeled in ( a , b ) and p values by two-way ANOVA are labeled in ( f – i ).

Article Snippet: Sera were immediately isolated, sub-bottled, and stored at −80 °C until being assayed using Human BMP6 and BMP2 ELISA kit (LSBio, LS-F4538; R&D, DBP200).

Techniques: RNA Sequencing Assay, Transfection, Transwell Assay, Tube Formation Assay, Migration, Two Tailed Test, Labeling

Journal: eBioMedicine

Article Title: H3K27me3-modulated Hofbauer cell BMP2 signalling enhancement compensates for shallow trophoblast invasion in preeclampsia

doi: 10.1016/j.ebiom.2023.104664

Figure Lengend Snippet: BMP2 upregulates BMP6 in trophoblast via BMPR1A-SMAD2/3-SMAD4 signalling . a, HTR8/SVneo cells were treated with or without 25 ng/mL BMP2 for the indicated durations and the protein levels of targets were assayed by immunoblotting. b , HTR8/SVneo cells were pretreated with vehicle (DMSO), ACVR1 and BMPR1A inhibitor DMH-1 (1 μM), or ACVR1B, TGFβRI and ACVR1C inhibitor SB431542 (10 μM) for 60 min followed by treatment with or without BMP2 (25 ng/mL) for 1 h (upper panel) or 48 h (lower panel). The protein levels of targets were assayed by immunoblotting. c-d , HTR8/SVneo cells were transfected with 20 nM non-targeting control siRNA or siRNA targeting ACVR1B and TGFBR1 ( c ) or ACVR1 and BMPR1A ( d ) for 48 h prior to treatment with or without BMP2 (25 ng/mL) for 1 h (upper panel) or 48 h (lower panel). The protein levels of targets were assayed by immunoblotting. e , g and h , Two published single cell transcriptomes of placentas from 5 preeclamptic donors and 5 healthy donors were integrated and re-analyzed. e , t-SNE plot (left panel) and violin plot (right panel) displays the BMPR1A expression level in all cell types. g , Violin plot displays the expression level of PE related DEGs in BMPR1A + VCTs and BMPR1A − VCTs. h , Dot plots displays the significantly enriched gene sets using the DEGs in g . Dot size represents the number of DEGs from a particular gene set (count). f , Immunofluorescence staining of E-cadherin and BMPR1A in first-trimester placental villi. The lower panel is higher powered images of the upper panel. VCT, villous cytotrophoblast; SCT, syncytiotrophoblast; CCC, cytotrophoblast cell column. Scale bar, 40 μm.

Article Snippet: Sera were immediately isolated, sub-bottled, and stored at −80 °C until being assayed using Human BMP6 and BMP2 ELISA kit (LSBio, LS-F4538; R&D, DBP200).

Techniques: Western Blot, Transfection, Expressing, Immunofluorescence, Staining

Journal: eBioMedicine

Article Title: H3K27me3-modulated Hofbauer cell BMP2 signalling enhancement compensates for shallow trophoblast invasion in preeclampsia

doi: 10.1016/j.ebiom.2023.104664

Figure Lengend Snippet: H3K27me3-modulated Hofbauer cell BMP2 signalling enhancement compensates for shallow trophoblast invasion in preeclampsia . By taking advantage of clinical samples from healthy and preeclamptic pregnant participants, as well as in vitro , ex vivo and in vivo experimental models, we found that BMP2, a pro-invasive factor of human trophoblast, was upregulated in preeclamptic placentas with Hofbauer cells as its cellular origin; Reduced H3K27me3 modification contributes to the observed BMP2 upregulation in preeclamptic placentas; BMP6, a downstream target of BMP2 and a newly identified pro-invasive factor in trophoblasts, was upregulated in late gestational serum of patients with preeclampsia; BMP2 promotes trophoblast invasion and vascular mimicry by upregulating BMP6 expression in a BMPR1A-SMAD2/3-SMAD4-dependent manner. Our findings demonstrate that epigenetically regulated Hofbauer cell-derived BMP2 signalling enhancement in late gestation could serve as a compensatory response for shallow trophoblast invasion in PE, suggesting opportunities for diagnostic marker and therapeutic target applications in PE clinical management. This schematic diagram is created with Biorender.com .

Article Snippet: Sera were immediately isolated, sub-bottled, and stored at −80 °C until being assayed using Human BMP6 and BMP2 ELISA kit (LSBio, LS-F4538; R&D, DBP200).

Techniques: In Vitro, Ex Vivo, In Vivo, Modification, Expressing, Derivative Assay, Diagnostic Assay, Marker